SG-APSIC1203: Detection of carbapenemase genes in donor lungs at the point of care before transplantation reduces the risk of carbapenem-resistant Enterobacteriaceae–associated donor-derived infection in lung-transplant recipients

Objectives: The number of lung transplants is increasing year by in China and globally. With the widespread use donation after brainstem death (DBD) donor lungs circulatory (DCD) lungs, donor-derived infection (DDI) poses a major challenge transplantation. Using infected or colonized with carbapenem-resistant Enterobacteriaceae (CRE) may have serious implications lung-transplant recipients. Currently, traditional microbial culture along antimicrobial susceptibility testing cannot fully meet need for rapid accurate diagnosis CRE before organ harvest. Methods: Xpert Carba-R device (Cepheid, Sunnyvale, CA) was used to detect differentiate Klebsiella pneumoniae carbapenemase (KPC), New Delhi metallo-β-lactamase (NDM), Verona integron-encoded (VIM), active-on-imipenem (IMP), OXA-48 genotypes bronchial lavage fluid from Positive detection 1 more these indicated potentially CRE-infected lung, organs were removed transplantation cohort. Donor negative all KPC, NDM, VIM, IMP, determined incidence CRE-associated DDI infection-related complications compared screening group an historic control group. Results: In this study, 21 tested genotypes. Among them, 4 positive discarded, remaining 17 showing no gene presence No occurred Conclusions: Rapid at point care using reduced risk